http://nickfisklab.org/aboutnick daily 0.75 2023-08-21 https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1479317177322-W8D3F55SXMCVOX2XBPI9/image-asset.jpeg About Nick Fisk https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1482869327156-ANDLZT51Q8DQCNNV3BST/image-asset.jpeg About Nick Fisk https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470958218734-W8GSFZARDB1JDSCJGGI1/RMNP-panorama1.JPG About Nick Fisk http://nickfisklab.org/research daily 0.75 2016-10-10 https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470947002828-FU2N5BBPKNJJ9NVVK63C/image-asset.png Research The Fisk Lab's fluorescence-based screen for sense codon reassignment. Our screen evaluates the ability of anticodon-modified tRNAs to incorporate tyrosine (Tyr) in response to a sense codon that is assigned another identity in the standard E. coli genetic code. Residues 65-67 of superfolder GFP specify the Thr-Tyr-Gly sequence that autocatalytically folds into the tripeptide fluorophore. Replacement of Tyr at position 66 with any other natural amino acid effectively abolishes the fluorescence of the protein. (Biochemistry, 2015, 54 (50), pp 7355–7364) https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470948364318-Y45L8U82Q7X4BR2JBAOT/Figure00_SimpleTOC_v02.png Research The Fisk lab is interested in exploiting the self-replicating, self-assembling M13 phage particle as a platform for multivalent, mulifunctional biotechnologies. This goal requires a complete understanding of the quantitative biology involved in the entire M13 life cycle. Our first two papers developing and utilizing a chemical kinetic simulation of the entire M13 life cycle have been published. (Virology, 2016, http://dx.doi.org/10.1016/j.virol.2016.08.017 and http://dx/doi.org/10.1016/j.virol.2016.08.015) https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470947994791-ZZQ5BLQPG06GNS4U2454/image-asset.jpeg Research The Fisk lab is interested in identifying new scaffold proteins for phage display libraries. We have had success identifying tight, specific binding molecules for a variety of protein targets from a library based on the 65 amino acid hyperthermophilic scaffold protein Sso7d. (FEBS J, 2016, 283, pp 1351–1367) https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470956859779-9D3NNHVODND2ZWZ5TCSG/RMNP-panorama6.JPG Research http://nickfisklab.org/contact daily 0.75 2018-06-14 https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470954655667-2KI5K2FYIWSK2FUVC2CM/P1040794.JPG Contact http://nickfisklab.org/welcome daily 1.0 2018-06-14 https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470953592968-38ZL8ZNDJ7BX1LOZZ6Y0/RMNP-panorama4.JPG Welcome http://nickfisklab.org/news daily 0.75 2024-08-05 https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1661799589719-4JGLE7N8PVQQ3C2O3AVC/2022_Commencement_IMG_7974.jpg News https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1661799755610-8OO6MT34I0JBRQ01YMR1/Commencement_2022_IMG_8006.jpg News https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1661799754431-PAPF0RAAD1MQ1PA54MO7/Commencement+2022_IMG_7995.jpg News https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1661963123398-PCZZYF0M0YGO85TB9AKN/VideoCapture_20220822-111618.jpg News https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1661963183588-MZEEN2N62BOLKSJ00DJ4/20220820_104136.jpg News https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1661963172061-94A9IYXVABGZDRXONUBP/VideoCapture_20220822-113857.jpg News https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470957100140-3NSGIPRDLV99APEIFSMB/RMNP-panorama7.JPG News http://nickfisklab.org/people daily 0.75 2023-08-21 https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470948897098-QAGXPAL1WBT75QIULQ5Q/image-asset.jpeg People https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470958026147-WJXMLF5VHW4FDVGSOY1Q/20150407+Fisk+Lab+Group+Photo_resize_2.jpg People http://nickfisklab.org/publications daily 0.75 2024-08-05 https://images.squarespace-cdn.com/content/v1/57acb061ebbd1aa31e43b8b7/1470956963825-UY5V3962XMMEDC61T867/RMNP-panorama4.JPG Publications http://nickfisklab.org/read-me daily 0.75 2025-05-12 https://images.squarespace-cdn.com/content/v1/5052176b84aeb45fa5cfcc83/1377096507573-1MU42OOOOGIM9URAJ9D9/515c4225e4b0afec1218b21e.png Read Me https://images.squarespace-cdn.com/content/v1/5052176b84aeb45fa5cfcc83/1377096775448-0L7B4Q54H1VLOGFF1DPR/synthetica+%2820130821%29.jpg Read Me https://images.squarespace-cdn.com/content/v1/5052176b84aeb45fa5cfcc83/1377097104716-HDI53XJVV0DKZ24A3T68/51e453dee4b06abd3403c22e.png Read Me